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The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline

[25] In human cells with mutant WRN, NAD+ repletion restored normal mitophagy, improved fat metabolism, decreased mitochondrial oxidative stress, and enhanced mitochondrial quality

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Similar content being viewed by others Background Intestinal ischemia/reperfusion injury (IIRI) is a life-threatening condition that results from sudden deprivation of intestinal blood flow and systemic diseases, such as incarcerated hernia [1], intestinal transplantation [2], hemorrhagic shock [3], and sepsis [4]